RESUMO
Objetivo: analisar o polimorfismo genético do fator de necrose tumoral alfa (TNF-α -308G/A) em idosos com hipertensão arterial. Método: estudo quantitativo, descritivo e transversal realizado com 130 idosos em uma Unidade Básica de Saúde do Distrito Federal. A coleta de dados iniciou com coleta de sangue para análises das concentra- ções de triglicerídeos, HDL, LDL, colesterol total, glicemia de jejum e níveis de fator de necrose tumoral alfa (TNF-α). Foi mensurada a pressão arterial, investigado dados sociodemográficos, hábitos de vida e dados antropométricos. A reação em cadeia da polimerase (PCR) foi padronizada e realizada para os genes do TNF-α. A análise estatística foi realizada no SPSS 20.0. Resultados: A idade elevada (p=0,007), aposentadoria (p=0,024) e tabagismo (p=0,019) foram significativamente relacionados à hipertensão arterial. Os idosos hipertensos apresentaram menores níveis de HDL (p=0,013) e maiores expressões de TNF-α (p=0,025). Uma frequência maior para o genó- tipo de homozigotos GG (61,8%) foi observada nos normotensos. Por outro lado, nos heterozigotos 54,7% apresentaram genótipo GA. Os idosos que possuíram o genótipo AA demonstraram 2,87 vezes mais chances de terem HAS. Conclusão: Esse achado tem importância clínica no que diz respeito ao manejo da hipertensão e de outras doenças crônicas, pois o controle da inflamação poderia reduzir a variação da pressão arterial e de suas consequências cardiovasculares.(AU)
Objective: to analyze the genetic polymorphism of the tumor necrosis factor alpha (TNFα -308 G/A) in elderly people with arterial hypertension. Method: a quantitative, descriptive and cross-sectional study carried out with 130 elderly people in a Basic Health Unit in the Federal District. Data gathering started with blood collection for analysis of triglyceride concentrations, HDL, LDL, total cholesterol, fasting glucose and levels of tumor necrosis factor alpha (TNFα). Blood pressure was measured, and sociodemographic data, life style and anthropometric data were investigated. The polymerase chain reaction (PCR) was standardized and performed for TNFα genes. Statistical analysis was performed using SPSS 20.0. Results: High age (p = 0.007), retirement (p = 0.024), and smoking (p = 0.019) were significantly related to arterial hypertension. Hypertensive elderly people had lower HDL levels (p = 0.013) and higher expressions of TNFα (p = 0.025). A higher frequency for the GG homozygous genotype (61.8%) was observed in normotensive individuals. On the other hand, in the heterozygotes, 54.7% had GA genotype. The elderly who had the AA genotype were 2.87 times more likely to have arterial hypertension. Conclusion: This finding is of clinical importance with regard to the management of hypertension and other chronic disease, as the control of inflammation could reduce the variation in blood pressure and its cardiovascular consequences.(AU)
Assuntos
Polimorfismo Genético , Idoso , Linfotoxina-alfa , HipertensãoRESUMO
Objectives Oral lichen planus (OLP) is a chronic inflammatory oral mucosal disease. Cytokines play an important role in the pathogenesis and disease progression of OLP. Various reports have implicated cytokine gene polymorphisms in susceptibility to develop some immune mediated conditions including OLP. The purpose of this study was to investigate the association of tumor necrosis factor (TNF)-α, TNF-β and interleukin (IL)-10 gene polymorphisms with the OLP risk. Material and Methods Forty two unrelated patients with OLP and 211 healthy volunteers were genotyped for TNF-α (-308 G/A), TNF-β (+252A/G), IL-10 (-1082G/A), IL-10 (-819C/T), and IL-10 (-592C/A) polymorphisms. Results The frequencies of allele A and genotype GA of TNF-α (-308G/A) were significantly higher while allele G and GG genotypes were lower in OLP patients as compared to the controls (P<0.001). The frequency of GA genotype of TNF-β (+252A/G) was significantly higher in patients than in controls while the AA genotype was completely absent in OLP patients. These results indicated that allele A and genotype GA of TNF-α (-308G/A) as well as the GA genotype of TNF-β (+252A/G) polymorphisms are associated with OLP risk. The frequencies of alleles and genotypes of -1082G/A, -819C/T and -592C/A polymorphisms in IL-10 gene did not differ significantly between OLP patients and controls (P>0.05). However, haplotype ATA extracted from 1082G/A, -819C/T, -592C/A polymorphisms of IL-10 were more prevalent in OLP patients when compared to controls indicating its possible association with OLP susceptibility. Conclusion It is concluded that TNF-α (-308G/A), TNF-β (+252A/G) and IL-10 (-1082G/A, -819C/T and -592C/A) polymorphisms are associated with the susceptibility of OLP, thus giving additional support for the genetic basis of this disease. .
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , /genética , Líquen Plano Bucal/genética , Linfotoxina-alfa/genética , Polimorfismo de Nucleotídeo Único/genética , Fator de Necrose Tumoral alfa/genética , Estudos de Casos e Controles , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença/genética , Líquen Plano Bucal/patologia , Reação em Cadeia da Polimerase , Valores de Referência , Fatores de Risco , Arábia Saudita , Fatores SexuaisRESUMO
Advances in diagnostic research are moving towards methods whereby the periodontal risk can be identified and quantified by objective measures using biomarkers. Patients with periodontitis may have elevated circulating levels of specific inflammatory markers that can be correlated to the severity of the disease. The purpose of this study was to evaluate whether differences in the serum levels of inflammatory biomarkers are differentially expressed in healthy and periodontitis patients. Twenty-five patients (8 healthy patients and 17 chronic periodontitis patients) were enrolled in the study. A 15 mL blood sample was used for identification of the inflammatory markers, with a human inflammatory flow cytometry multiplex assay. Among 24 assessed cytokines, only 3 (RANTES, MIG and Eotaxin) were statistically different between groups (p<0.05). In conclusion, some of the selected markers of inflammation are differentially expressed in healthy and periodontitis patients. Cytokine profile analysis may be further explored to distinguish the periodontitis patients from the ones free of disease and also to be used as a measure of risk. The present data, however, are limited and larger sample size studies are required to validate the findings of the specific biomarkers.
Avanços no diagnóstico da doença periodontal levam a métodos nos quais o risco e atividade da doença periodontal podem ser identificados e quantificados por biomarcadores. Pacientes com periodontite podem apresentar elevados níveis circulatórios de marcadores inflamatórios específicos que podem ser correlacionados com a severidade da doença. Portanto, o objetivo desse estudo foi avaliar as diferenças nos níveis séricos de biomarcadores inflamatórios em pacientes saudáveis e com doença periodontal. Foram incluídos no estudo 25 pacientes (8 saudáveis e 17 com periodontite crônica). Uma amostra de 15 mL de sangue foi obtida para identificar os marcadores inflamatórios simultaneamente utilizando Array de proteínas através de citometria de fluxo. De 24 citocinas inflamatórias analisadas, apenas 3 (RANTES, MIG e Eotaxina) apresentaram diferenças estatisticamente significantes (p<0,05) entre os dois grupos. Conclui-se que alguns marcadores inflamatórios selecionados apresentam diferença de concentração em pacientes com periodontite e saudáveis. A análise do perfil de citocinas pode ser utilizada tanto para distinguir pacientes periodontais de pacientes saudáveis, como para medir o risco à doença. Contudo, mais estudos com número maior de amostras são necessários para validar os achados sobre os biomarcadores específicos.